Middle-aged healthy women and Alzheimer's disease patients present an overlapping of brain cell transcriptional profile. (15th May 2019)

• Record Type: Journal Article
• Authors: Sanfilippo, Cristina; Castrogiovanni, Paola; Imbesi, Rosa; Tibullo, Daniele; Li Volti, Giovanni; Barbagallo, Ignazio; Vicario, Nunzio; Musumeci, Giuseppe; Di Rosa, Michelino
• Is Part Of: Neuroscience. Volume 406(2019)
• Page Start: 333
• Page End: 344
• Publication Date: 2019-05-15
• Subjects: PET positron emission tomography -- fMRI functional magnetic resonance imaging -- SDEG significantly different expressed genes -- LOAD late onset Alzheimer disease -- PFC dorsolateral prefrontal cortex -- VC visual cortex -- CR cerebellum -- HBTRC Harvard brain tissue resource center -- GEO gene expression omnibus -- GO gene ontology -- RNA-seq. RNA-sequencing -- MeV MultiExperiment Viewer -- GATHER Gene Annotation Tool to Help Explain Relationships -- WPSEG weighted percentages of significantly expressed genes -- RF representation factor -- CNS central nervous system -- OLIGO oligodendrocytes -- NEU neuron -- ASTRO astrocytes -- ENDO endothelial cells or endotheliocyte -- MICRO microglia -- DENDR-DEV dendrite development -- MICRO-ACT microglial cell activation -- MONO Monocyte -- MACRO Macrophage -- CD4 cluster of differentiation 4 -- CD8 cluster of differentiation 8 -- SYNAP synaptic transmission genes -- AD Alzheimer's disease -- Up up-regulated genes -- Down down-regulated genes -- neg ln negative logarithm natural -- aa average ages; Aging -- Alzheimer's disease -- PFC -- Microglia -- Microarray; Neurochemistry -- Periodicals; Neurophysiology -- Periodicals; Neurology -- Periodicals; Neurochimie -- Périodiques; Neurophysiologie -- Périodiques; Neurochemistry; Neurophysiology; Electronic journals; Periodicals; Electronic journals; 612.8
• DOI: 10.1016/j.neuroscience.2019.03.008
• Languages: English
• ISSNs: 0306-4522

Abstract

Abstract: Exploring sexual dimorphisms in the brain morphology is important for their impact and therapeutic implications for several neurological diseases. The hypothesis that sex could influence the transcriptome of brain cells could be the basis regarding the different response to cognitive decline identified in men and women. In this paper, we analyzed several prefrontal cortices (PFC) microarrays datasets of young/middle-aged healthy subjects and then Alzheimer's disease (AD) patients, according to the sex. The significant transcriptomes were overlapped with the main genes characterizing cells of the central nervous system (CNS) in order to determine the respective weighted percentages of significantly expression gene modulation (WPSEG). We identified differences in brain transcriptional activity between young and middle-aged. In middle-aged women, the WPSEG were higher for the Astrocytes, the Endotheliocytes, and the Microglia. In addition, the sex-matched analysis of transcriptome identified a convergent molecular signature in men and women AD patients. Furthermore, the WPSEG belonging to CNS cells in PFC of healthy middle-aged subjects was correlated to AD profiles according to the sex. Since our results, it is possible to conclude that during the aging the PFC' cells adopt transcriptional strategies sex-dependent that could potentially control the development of neurodegenerative diseases. Graphical Abstract: Unlabelled Image Highlights: There is a difference proportion of gray matter in human men and women. Online microarray datasets could be used to identify the brain cells transcriptome difference between men and women. There are substantial differences in brain transcriptional activity between young and middle-aged men and women. The transcriptome sex-matched analysis identified a convergent molecular signature in AD men and women patients.