Degalactotigonin, a Steroidal Glycoside from Solanum nigrum, Induces Apoptosis and Cell Cycle Arrest via Inhibiting the EGFR Signaling Pathways in Pancreatic Cancer Cells. (16th December 2018)

• Record Type: Journal Article
• Authors: Tuan Anh, Hoang Le; Tran, Phuong Thao; Thao, Do Thi; Trang, Duong Thu; Dang, Nguyen Hai; Van Cuong, Pham; Kiem, Phan Van; Minh, Chau Van; Lee, Jeong-Hyung
• Other Names: Forni Cinzia Guest Editor.
• Is Part Of: BioMed research international. Volume 2018(2018)
• Publication Date: 2018-12-16
• Subjects: Medicine -- Periodicals; Biology -- Periodicals; Biotechnology -- Periodicals; Life sciences -- Periodicals; 610.5
• DOI: 10.1155/2018/3120972
• Languages: English
• ISSNs: 2314-6133

Abstract

Abstract : Degalactotigonin (1 ) and three other steroidal compounds solasodine (2 ), O -acetyl solasodine (3 ), and soladulcoside A (4 ) were isolated from the methanolic extract of Solanum nigrum, and their chemical structures were elucidated by spectroscopic analyses. The isolated compounds were evaluated for cytotoxic activity against human pancreatic cancer cell lines (PANC1 and MIA-PaCa2) and lung cancer cell lines (A549, NCI-H1975, and NCI-H1299). Only degalactotigonin (1 ) showed potent cytotoxicity against these cancer cell lines. Compound1 induced apoptosis in PANC1 and A549 cells. Further study on its mechanism of action in PANC1 cells demonstrated that1 significantly inhibited EGF-induced proliferation and migration in a concentration-dependent manner. Treatment of PANC1 cells with degalactotigonin induced cell cycle arrest at G0/G1 phase. Compound1 induced downregulation of cyclin D1 and upregulation of p21 in a time- and concentration-dependent manner and inhibited EGF-induced phosphorylation of EGFR, as well as activation of EGFR downstream signaling molecules such as Akt and ERK.