Genome‐wide analysis of DNA methylation in endometriosis using Illumina Human Methylation 450 K BeadChips. Issue 5 (11th February 2019)

• Record Type: Journal Article
• Authors: Wang, Lixian; Zhao, Jian; Li, Yan; Wang, Zihe; Kang, Shan
• Is Part Of: Molecular reproduction and development. Volume 86:Issue 5(2019)
• Page Start: 491
• Page End: 501
• Publication Date: 2019-02-11
• Subjects: DNA methylation -- DNA methyltransferase enzymes (DNMTs) -- endometriosis -- epigenetic modifications -- methyl‐CpG‐binding domain protein 2 (MBD2); Reproduction -- Periodicals; Molecular biology -- Periodicals; Molecular genetics -- Periodicals; Embryology -- Periodicals; 571.8
• DOI: 10.1002/mrd.23127
• Languages: English
• ISSNs: 1040-452X

Abstract

Abstract: Endometriosis is a common chronic gynecologic disorder characterized by the presence and growth of endometrial‐like tissue outside of the uterine cavity. Although the exact etiology remains unclear, epigenetic modifications, such as DNA methylation, are thought to contribute to the pathogenesis of endometriosis. Here, we used the Illumina Human Methylation 450 K BeadChip Array to analyze the genome‐wide DNA methylation profiles of six endometriotic lesions and six eutopic endometria from patients with ovarian endometriosis and six endometria of women without endometriosis. Compared with the eutopic endometria of women with endometriosis, 12, 159 differentially methylated CpG sites and 375 differentially methylated promoter regions were identified in endometriotic lesions. GO analyses showed that these putative differentially methylated genes were primarily associated with immune response, inflammatory response, response to steroid hormone stimulus, cell adhesion, negative regulation of apoptosis, and activation of the MAPK activity. In addition, the expression levels of DNMT1, DNMT3A, DNMT3B, and MBD2 in endometriotic lesions and eutopic endometria were significantly decreased compared with control endometria. Our findings suggest that aberrant DNA methylation status in endometriotic lesions may play a significant role in the pathogenesis and progression of endometriosis. Abstract : In the present study, we used the Infinium Human Methylation 450 K BeadChip Array to analyze DNA methylation status in endometriotic lesions and eutopic endometria of the same patients with ovarian endometriosis and the endometria of women without endometriosis. The data showed that significant methylation differences existed in endometriotic lesions compared with eutopic and control endometria.