Harnessing Human Decellularized Blood Vessel Matrices and Cellular Construct Implants to Promote Bone Healing in an Ex Vivo Organotypic Bone Defect Model. Issue 9 (14th May 2018)

• Record Type: Journal Article
• Authors: Inglis, Stefanie; Schneider, Karl Heinrich; Kanczler, Janos M.; Redl, Heinz; Oreffo, Richard O. C.
• Is Part Of: Advanced healthcare materials. Volume 8:Issue 9(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-05-14
• Subjects: cell constructs -- decellularized matrices -- drill defects -- ex vivo -- organotypic cultures; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/adhm.201800088
• Languages: English
• ISSNs: 2192-2640

Abstract

Abstract: Decellularized matrices offer a beneficial substitute for biomimetic scaffolds in tissue engineering. The current study examines the potential of decellularized placental vessel sleeves (PVS) as a periosteal protective sleeve to enhance bone regeneration in embryonic day 18 chick femurs contained within the PVS and cultured organotypically over a 10 day period. The femurs are inserted into decellularized biocompatibility‐tested PVS and maintained in an organotypic culture for a period of 10 days. In femurs containing decellularized PVS, a significant increase in bone volume ( p < 0.001) is evident, demonstrated by microcomputed tomography (µCT) compared to femurs without PVS. Histological and immunohistological analyses reveal extensive integration of decellularized PVS with the bone periosteum, and enhanced conservation of bone architecture within the PVS. In addition, the expressions of hypoxia inducible factor‐1 alpha (HIF‐1α), type II collagen (COL‐II), and proteoglycans are observed, indicating a possible repair mechanism via a cartilaginous stage of the bone tissue within the sleeve. The use of decellularized matrices like PVS offers a promising therapeutic strategy in surgical tissue replacement, promoting biocompatibility and architecture of the tissue as well as a factor‐rich niche environment with negligible immunogenicity. Abstract : Decellularized tissue matrices have become a beneficial substitute for biomimetic scaffold in tissue engineering. The current study has examined decellularized placental vascular sleeves (PVS) as a periosteal protective sleeve to enhance the repair of a bone defect. PVS femurs in organotypic culture augment bone healing and produce higher levels of bone formation, offering the potential for future bone regenerative strategies.