Tc1/Tc2 ratio in the inflammatory process in patients with Behçet's disease. Issue 4 (2004)

• Record Type: Journal Article
• Authors: Houman, Habib; Hamzaoui, Agnes; Ghorbal, Imed Ben; Khanfir, Monia Smiti; Feki, Moncef; Hamzaoui, Kamel
• Is Part Of: Mediators of inflammation. Volume 13:Issue 4(2004)
• Page Start: 247
• Page End: 253
• Publication Date: 2004
• Subjects: Behçet's disease -- CD8 T cells -- Inflammation -- MIP-1α -- Human microvascular endothelial cells; Inflammation -- Mediators -- Periodicals; Biological response modifiers -- Periodicals; Inflammation (Pathologie) -- Médiateurs; Immunomodulateurs; Biological response modifiers; Inflammation -- Mediators; Immunology; Autacoids; Immunologic Factors; Cell Adhesion Molecules; Cell Communication; Cytokines; Inflammation; Periodicals; Electronic journals; 616.0473
• DOI: 10.1080/09629350400003167
• Languages: English
• ISSNs: 0962-9351

Abstract

Abstract : BACKGROUND : Peripheral blood CD8 + T cells expressing interferon gamma and interleukin-4 (IL-4), and lacking CD28 molecules, were responsible for the dynamic interplay between peripheral blood and inflammatory sites. Introduction : The aim of the current study was to define in Behçet's disease (BD), CD8 + T-cell subsets using CD28 and CD11b monoclonal antibodies, and the characterization of the Tc1/Tc2 ratio and perforin expression. Methods : Flow cytometry was used for intracytoplasmic cytokines and perforin expression. Effector cells were investigated by adhesion of CD8 + T cells to human microvascular endothelial cells and by chemotaxis using β-chemokine. Results : Interferon-gamma-producing CD8 + T cells in active and remission BD patients were increased, which induce a significant increase of the Tc1:Tc2 ratio in BD. CD8 + CD28 − CD11b + T cells were found to be more expanded in BD patients than in age-matched healthy controls. The expression of CD11b molecules in active BD allowed to CD8 + CD28 + /CD8 + CD28 − subsets to adhere to human microvascular endothelial cells, with more efficiency in BD. Using MIP-1α, we observed that the migratory process of CD28 − CD11b + is more important in BD. CD28 − CD11b + exhibited an increased perforin expression in BD patients. Conclusion : Taken together these results suggest the presence of immune activation, probably in response to a profound inflammation affecting BD patients. The physiopathological significance of these results were toward autoimmune diseases and/or infectious process.