The effect of interleukin-15 on the expression of killer-cell immunoglobulin-like receptors on peripheral natural killer cells in human. Issue 4 (2002)

• Record Type: Journal Article
• Authors: Kogure, Toshiaki; Mantani, Naoki; Goto, Hirozo; Shimada, Yutaka; Tamura, Jun'ichi; Terasawa, Katsutoshi
• Is Part Of: Mediators of inflammation. Volume 11:Issue 4(2002)
• Page Start: 219
• Page End: 224
• Publication Date: 2002
• Subjects: Inflammation -- Mediators -- Periodicals; Biological response modifiers -- Periodicals; Inflammation (Pathologie) -- Médiateurs; Immunomodulateurs; Biological response modifiers; Inflammation -- Mediators; Immunology; Autacoids; Immunologic Factors; Cell Adhesion Molecules; Cell Communication; Cytokines; Inflammation; Periodicals; Electronic journals; 616.0473
• DOI: 10.1080/09629350290000078
• Languages: English
• ISSNs: 0962-9351

Abstract

Abstract : Interleukin (IL)-15 has emerged as a key regulator of both natural killer (NK) cell differentiation and activation. The aim of the present study was to investigate the expansion of the population of cells expressing killer-cell immunoglobulin-like receptors (CD158a and CD158b) in human peripheral lymphocytes by treatment with IL-15. One million peripheral lymphocytes were cultured in RPMI1640 medium alone or in medium containing IL-2 at 100 U/ml or IL-15 at 0.1, 1.0, or 10.0 ng/ml for 48 h. After each incubation, we assessed the natural killing activity and the population of CD16 + CD158a + /b + cells and CD8 + CD158a + /b + cells. IL-15 increased the NK activity and expanded the populations of CD16 + CD158a + /b + cells and CD8 + CD158a + /b + cells. These actions were dose dependent, and the effects of IL-15 at 1.0 ng/ml were close to those of IL-2 at 100 U/ml. These findings suggest that IL-15 induces the effector functions of resting NK cells throughout the body, and thereby plays a critical role in the activation of tissue-associated immune responses.