Complete genome of Vibrio parahaemolyticus FORC014 isolated from the toothfish. Issue 1 (December 2016)

• Record Type: Journal Article
• Authors: Ahn, Sojin; Chung, Han Young; Lim, Sooyeon; Kim, Kwondo; Kim, Suyeon; Na, Eun Jung; Caetano-Anolles, Kelsey; Lee, Ju-Hoon; Ryu, Sangryeol; Choi, Sang Ho; Kim, Heebal
• Is Part Of: Gut pathogens. Volume 8:Issue 1(2016)
• Page Start: 1
• Page End: 6
• Publication Date: 2016-12
• Subjects: Vibrio parahaemolyticus -- Type III secretion system-2 -- Whole genome sequencing -- Comparative genomics; Gastrointestinal system -- Microbiology -- Periodicals; 616.3
• DOI: 10.1186/s13099-016-0134-0
• Languages: English
• ISSNs: 1757-4749

Abstract

Abstract Background Foodborne illness can occur due to various pathogenic bacteria such asStaphylococcus aureus, Escherichia coli andVibrio parahaemolyticus, and can cause severe gastroenteritis symptoms. In this study, we completed the genome sequence of a foodborne pathogenV. parahaemolyticus FORC_014, which was isolated from suspected contaminated toothfish from South Korea. Additionally, we extended our knowledge of genomic characteristics of the FORC_014 strain through comparative analysis using the complete sequences of otherV. parahaemolyticus strains whose complete genomes have previously been reported. Results The complete genome sequence ofV. parahaemolyticus FORC_014 was generated using the PacBio RS platform with single molecule, real-time (SMRT) sequencing. The FORC_014 strain consists of two circular chromosomes (3, 241, 330 bp for chromosome 1 and 1, 997, 247 bp for chromosome 2), one plasmid (51, 383 bp), and one putative phage sequence (96, 896 bp). The genome contains a total of 4274 putative protein coding sequences, 126 tRNA genes and 34 rRNA genes. Furthermore, we found 33 type III secretion system 1 (T3SS1) related proteins and 15 type III secretion system 2 (T3SS2) related proteins on chromosome 1. This is the first reported result of Type III secretion system 2 located on chromosome 1 ofV. parahaemolyticus without thermostable direct hemolysin (tdh ) and thermostable direct hemolysin-related hemolysin (trh ). Conclusions Through investigation of the complete genome sequence ofV. parahaemolyticus FORC_014, which differs from previously reported strains, we revealed two type III secretion systems (T3SS1, T3SS2) located on chromosome 1 which do not includetdh andtrh genes. We also identified several virulence factors carried by our strain, including iron uptake system, hemolysin and secretion system. This result suggests that the FORC_014 strain may be one pathogen responsible for foodborne illness outbreak. Our results provide significant genomic clues which will assist in future understanding of virulence at the genomic level and help distinguish between clinical and non-clinical isolates.