Complete genome sequence of Vibrio vulnificus FORC_017 isolated from a patient with a hemorrhagic rash after consuming raw dotted gizzard shad. Issue 1 (December 2016)

• Record Type: Journal Article
• Authors: Chung, Han; Kim, You-Tae; Kim, Suyeon; Na, Eun; Ku, Hye-Jin; Lee, Keun; Heo, Sang; Ryu, Sangryeol; Kim, Heebal; Choi, Sang; Lee, Ju-Hoon
• Is Part Of: Gut pathogens. Volume 8:Issue 1(2016)
• Page Start: 1
• Page End: 6
• Publication Date: 2016-12
• Subjects: Vibrio vulnificus -- Genome sequencing -- Hybrid assembly -- Pathogenesis -- Virulence factor -- Hemolysin -- Secretion system; Gastrointestinal system -- Microbiology -- Periodicals; 616.3
• DOI: 10.1186/s13099-016-0104-6
• Languages: English
• ISSNs: 1757-4749

Abstract

Abstract Background Vibrio vulnificus, a resident in the human gut, is frequently found in seafood, causing food-borne illnesses including gastroenteritis and severe septicemia. WhileV. vulnificus has been known to be one of the major food-borne pathogens, pathogenicity and virulence factors are not fully understood yet. To extend our understanding of the pathogenesis ofV. vulnificus at the genomic level, the genome ofV. vulnificus FORC_017 isolated from a female patient experiencing a hemorrhagic rash was completely sequenced and analyzed. Results Three discontinuous contigs were generated from a hybrid assembly using Illumina MiSeq and PacBio platforms, revealing that the genome of the FORC_017 consists of two circular chromosomes and a plasmid. Chromosome I consists of 3, 253, 417-bp (GC content 46.49 %) containing 2943 predicted open reading frames (ORFs) and chromosome II of 1, 905, 745-bp (GC content 46.90 %) containing 1638 ORFs. The plasmid pFORC17 consists of 70, 069-bp (GC content 43.77 %) containing 84 ORFs. The average nucleotide identity (ANI) value of the FORC_017 and CMCP6 strains was 98.53, suggesting that they are closely related. Conclusions Pathogenesis-associated genes includingvvhA, rtx gene cluster, and various hemolysin genes were present in FORC_017. In addition, three complete secretion systems (Type I, II and VI) as well as iron uptake-related genes for virulence of the FORC_017 were detected, suggesting that this strain is pathogenic. Further comparative genome analysis revealed that FORC_017 and CMCP6 share major toxin genes includingvvhA andrtx for pathogenesis activities. The genome information of the FORC_017 provides novel insights into pathogenicity and virulence factors ofV. vulnificus .