Increased urinary nitrite, a marker of nitric oxide, in active inflammatory bowel disease. Issue 2 (2001)

• Record Type: Journal Article
• Authors: Goggins, Michael G.; Shah, Syed A.; Goh, Jason; Cherukuri, Anil; Weir, Donal G.; Kelleher, Dermot; Mahmud, Nasir
• Is Part Of: Mediators of inflammation. Volume 10:Issue 2(2001)
• Page Start: 69
• Page End: 73
• Publication Date: 2001
• Subjects: Inflammation -- Mediators -- Periodicals; Biological response modifiers -- Periodicals; Inflammation (Pathologie) -- Médiateurs; Immunomodulateurs; Biological response modifiers; Inflammation -- Mediators; Immunology; Autacoids; Immunologic Factors; Cell Adhesion Molecules; Cell Communication; Cytokines; Inflammation; Periodicals; Electronic journals; 616.0473
• DOI: 10.1080/09629350120054536
• Languages: English
• ISSNs: 0962-9351

Abstract

Abstract : Background : Nitric oxide (NO) production is increased in inflammatory bowel disease (IBD), and measurement of NO metabolites may be useful for monitoring disease activity. Aims and objectives : To characterise urinary nitrite levels, a stable metabolite of NO, in IBD and to evaluate its potential as a marker of disease activity. Methods : Twelve-hour urinary nitrites were measured by the microplate assay method in 46 patients with IBD (active; n = 32). Urinary samples from 16 healthy individuals served as controls. Results : Increased levels of urinary nitrites were found in patients with active IBD compared with those with inactive IBD. Twenty-eight out of 32 patients (87.5%) with active IBD had detectable levels of nitrite in their urine as compared with 2/14 (14.3%) patients with inactive IBD. None of the 16 healthy controls had detectable urinary nitrite. Twelve-hour urinary nitrite in active compared with inactive IBD: 5 Ī 0.7 versus 0.1 Ī 0.04 μ mol( P < 0.05 ) . There was good correlation between urinary nitrite and some markers of disease activity in IBD such as C-reactive protein and microalbuminuria but not with erythrocyte sedimentation rate. Conclusions : Increased levels of nitrite were detected in urine of patients with active IBD, consistent with increased NO synthesis. This simple assay may be exploited as a potential marker of disease activity in IBD.