Isolation of villous cytotrophoblasts from second trimester human placentas. (15th December 2018)

• Record Type: Journal Article
• Authors: Jankovic-Karasoulos, Tanja; McAninch, Dale; McCullough, Dylan; Wilson, Rebecca L.; Bianco-Miotto, Tina; Roberts, Claire T.
• Is Part Of: Placenta. Volume 74(2019)
• Page Start: 55
• Page End: 58
• Publication Date: 2018-12-15
• Subjects: Trophoblasts -- Placenta -- Second trimester -- Primary cell isolation; Placenta -- Periodicals; Reproduction -- Periodicals; Placenta -- Periodicals; Placenta -- Périodiques; Reproduction -- Périodiques; 612.63
• DOI: 10.1016/j.placenta.2018.11.007
• Languages: English
• ISSNs: 0143-4004

Abstract

Abstract: Human placental functional studies are often performed using immortalised trophoblast cells and cell lines established from human choriocarcinomas, which, while practical, may not truly reflect trophoblast function in vivo . Primary trophoblast cultures derived from human placentas following pregnancy termination or delivery are more clinically relevant, but trophoblast isolation protocols are only available for 1st trimester and term placental tissues. Here we report a method for isolation and purification of primary villous cytotrophoblasts from 2nd trimester human placentas, that yields >99% trophoblast purity as shown by immunofluorescence. Highlights: 1 st trimester CTB isolation protocols are unsuitable for 2nd trimester tissue. Term placental CTB isolation protocols are also unsuitable for 2nd trimester tissue. Our protocol yields a highly pure CTB population from 2nd trimester placentas.