Zymosan-induced luminol-dependent chemiluminescence response of circulating and extravasated leukocytes in experimental sepsis. Issue 2 (2004)

• Record Type: Journal Article
• Authors: Khan, Haseeb Ahmad
• Is Part Of: Mediators of inflammation. Volume 13:Issue 2(2004)
• Page Start: 123
• Page End: 125
• Publication Date: 2004
• Subjects: Septicemia -- Peritonitis -- Leukocytes -- Phagocytosis -- Chemiluminescence -- Rat; Inflammation -- Mediators -- Periodicals; Biological response modifiers -- Periodicals; Inflammation (Pathologie) -- Médiateurs; Immunomodulateurs; Biological response modifiers; Inflammation -- Mediators; Immunology; Autacoids; Immunologic Factors; Cell Adhesion Molecules; Cell Communication; Cytokines; Inflammation; Periodicals; Electronic journals; 616.0473
• DOI: 10.1080/09629350410001688558
• Languages: English
• ISSNs: 0962-9351

Abstract

Abstract : This study examines a concurrent profiling of circulating and extravasated polymorphonuclear leukocytes (PMNs) in a rat model of experimental sepsis. Fecal peritonitis was induced in Wistar male rats by intraperitoneal instillation of a fecal suspension in saline (1:1 w/v). Blood and peritoneal fluid were collected 8 h following fecal inoculation for the evaluation of inflammatory response of PMNs using zymosan-induced luminol-dependent chemiluminescence. Fifty microliters of pre-diluted blood or peritoneal fluid samples were mixed with 150 μl of reaction mixture (4×10 −4 M luminol+50 μg opsonized zymosan+0.1% gelatin in Hank's balanced salt solution) and the chemiluminescence signal was measured in a luminometer at 37˚C. Fecal peritonitis caused a significant leukocytopenia (3540±297 mm−3 versus control value of 7525±711 mm −3, p < 0.001 ) accompanied by massive infiltration of PMNs in the peritoneal cavity (34700±4006 versus 7325±425 mm −3, p < 0.001 ). The phagocytic activity of circulating blood PMNs was down-regulated whereas a significant up-regulation was observed in the activity of PMNs from peritoneal fluid. In conclusion, this study clearly demonstrates sepsis-induced alterations in both blood and peritoneal fluid PMNs and their quantitative assessment may be helpful in disease evaluation and designing effective therapies.