A simple technique for establishing axenic cultures of centric diatoms from resting stage cells in bottom sediments. Issue 6 (1st November 2018)

• Record Type: Journal Article
• Authors: Ishii, Ken-Ichiro; Imai, Ichiro; Natsuike, Masafumi; Sawayama, Shigeki; Ishino, Ryota; Liu, Wen; Fukusaki, Koji; Ishikawa, Akira
• Is Part Of: Phycologia. Volume 57:Issue 6(2018)
• Page Start: 674
• Page End: 679
• Publication Date: 2018-11-01
• Subjects: Axenic culture -- Diatom -- Resting stage cell -- Vegetative cell; Algology -- Periodicals; Algae -- Periodicals; Algae; Natuurkunde; Algae; Periodicals; Electronic journals; 579.805
• DOI: 10.2216/17-73.1
• Languages: English
• ISSNs: 0031-8884

Abstract

Abstract: We devised a simple and rapid technique for establishing axenic cultures of centric diatoms by sieving and washing resting stage cells. This technique is an effective means for establishing the axenic cultures of species that have endogenous resting spores. The primary advantage of this method is in the isolation of cells when the target species is absent from the water column.