Development of a Molecular Marker for Fruiting Body Pattern in Auricularia auricula-judae. Issue 1 (2nd January 2018)

• Record Type: Journal Article
• Authors: Yao, Fang-Jie; Lu, Li-Xin; Wang, Peng; Fang, Ming; Zhang, You-Min; Chen, Ying; Zhang, Wei-Tong; Kong, Xiang-Hui; Lu, Jia; Honda, Yoichi
• Is Part Of: Mycobiology. Volume 46:Issue 1(2018)
• Page Start: 72
• Page End: 78
• Publication Date: 2018-01-02
• Subjects: Auricularia auricula-judae -- fruiting body pattern -- sequence-related amplified polymorphism -- sequence characterized amplified region; Mycology -- Periodicals; Mycology -- Korea -- Periodicals; Fungi -- Periodicals; Fungi -- Korea -- Periodicals; Fungi; Mycology; Korea; Mycology -- Periodicals; Fungi -- Periodicals; Periodicals; Electronic journals; 579.5
• DOI: 10.1080/12298093.2018.1454004
• Languages: English
• ISSNs: 1229-8093

Abstract

Abstract: The fruiting body pattern is an important agronomic trait of the edible fungus Auricularia auricula-judae, and an important breeding target. There are two types of fruiting body pattern: the cluster type and the chrysanthemum type. We identified the fruiting body pattern of 26 test strains, and then constructed two different near-isogenic pools. Then, we developed sequence characterized amplified region (SCAR) molecular markers associated with the fruiting body pattern based on sequence-related amplified polymorphism (SRAP) markers. Ten different bands (189–522 bp) were amplified using 153 pairs of SRAP primers. The SCAR marker "SCL-18" consisted of a single 522-bp band amplified from the cluster-type strains, but not the chrysanthemum strains. This SCAR marker was closely associated with the cluster-type fruiting body trait of A. auricula-judae . These results lay the foundation for further research to locate and clone genes controlling the fruiting body pattern of A. auricula-judae .