Iron regulatory protein is involved in the immune defense of the Chinese mitten crab Eriocheir sinensis. Issue 89 (June 2019)

• Record Type: Journal Article
• Authors: Yang, Lei; Li, Xuejie; Wu, Yaomeng; Zhang, Jiashun; Li, Weiwei; Wang, Qun
• Is Part Of: Fish & shellfish immunology. Issue 89(2019)
• Page Start: 632
• Page End: 640
• Publication Date: 2019-06
• Subjects: Eriocheir sinensis -- Iron regulatory protein -- Iron-responsive element -- Bacterial challenge; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2019.04.041
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Iron homeostasis is vital to organismal health; it is maintained by the iron regulatory protein (IRP)–iron-responsive element (IRE) signaling pathway. In the Chinese mitten crab Eriocheir sinensis, Es Fer-1 and Es Fer-2 reportedly have a putative IRE, but an IRP has not yet been identified. In this study, we successfully amplified the full-length cDNA of Es IRP using gene cloning and rapid amplification of cDNA ends techniques. The length of this cDNA was 4474 bp, and it included a 2682-bp open reading frame encoding 893 amino acids. Using quantitative real-time PCR, mRNA transcripts of Es IRP were detected in various tissues. The highest and lowest expression level was detected in the muscle and gills, respectively. In response to Staphylococcus aureus and Vibrio parahaemolyticus challenge, the transcription level of Es IRP was downregulated and that of Es Fer-1 and Es Fer-2 was upregulated in hemocytes. Es IRP knockdown resulted in increased expression of both Es Fer-1 and Es Fer-2. After Es Fer-1 and Es Fer-2 knockdown, the bacterial clearance ability of E. sinensis against S. aureus and V. parahaemolyticus was impaired. In conclusion, our results suggest that the IRP–IRE signaling pathway plays an important role in the innate immune system response in E. sinensis . Highlights: IRP–IRE signaling pathway has a key role in innate immunity in Eriocheir sinensis . Es IRP knockdown increases the expression of ferritins ( Es Fer-1 and Es Fer-2). Es Fer-1 and Es Fer-2 knockdown impairs bacterial clearance ability of E. sinensis .