MAVS of triploid hybrid of red crucian carp and allotetraploid possesses the improved antiviral activity compared with the counterparts of its parents. Issue 89 (June 2019)

• Record Type: Journal Article
• Authors: Xiao, Jun; Fu, Yongming; Wu, Hui; Chen, Xiangding; Liu, Shaojun; Feng, Hao
• Is Part Of: Fish & shellfish immunology. Issue 89(2019)
• Page Start: 18
• Page End: 26
• Publication Date: 2019-06
• Subjects: MAVS -- Triploid -- Hybridization -- Interferon -- SVCV -- GCRV; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2019.03.044
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Triploid hybrid (3n = 150) of red crucian carp (♀, 2n = 100) and allotetraploid (♂, 4n = 200) presents the obviously stronger disease resistance than its parents. To elucidate the innate immunity of triploid hybrid, the MAVS homologues of triploid hybrid (3nMAVS), red crucian carp (2nMAVS) and allotetraploid (4nMAVS) have been identified and characterized separately in this study. 2nMAVS and 4nMAVS were evolutionarily conserved; however, 3nMAVS showed lower amino acid similarity and differently predicted structure to 2nMAVS or 4nMAVS. 3nMAVS transcription increase rate in host cells were obviously higher than 2nMAVS or 4nMAVS in response to different stimuli, which included spring viraemia of carp virus (SVCV), grass carp reovirus (GCRV) and poly (I:C). The reporter assay in EPC cells showed that 3nMAVS owned much stronger ability to induce the production of DrIFNφ1 and eIFN than either 2nMAVS or 4nMAVS. Accordingly, EPC cells transfected with 3nMAVS presented obviously stronger antiviral activity against both GCRV and SVCV than the cells expressing 2nMAVS or 4nMAVS. All the data support the conclusion that 3nMAVS-mediated antiviral signaling during innate immune activation was stronger than those of 2nMAVS and 4nMAVS, which provided us the new insight on the innate immune system of triploid hybrid. Highlights: 3nMAVS presents lower amino acid similarity to 2nMAVS and 4nMAVS. The increase rate of 3nMAVS transcription in response to different stimuli is higher than that of 2nMAVS or 4nMAVS. 3nMAVS possesses much stronger antiviral activity than 2nMAVS and 4nMAVS.