Changes in the activities of antioxidant enzymes and reduced glutathione level in human erythrocytes exposed to selected brominated flame retardants. (July 2019)

• Record Type: Journal Article
• Authors: Jarosiewicz, Monika; Krokosz, Anita; Marczak, Agnieszka; Bukowska, Bożena
• Is Part Of: Chemosphere. Volume 227(2019)
• Page Start: 93
• Page End: 99
• Publication Date: 2019-07
• Subjects: Oxidative stress -- Antioxidant enzymes -- Brominated flame retardants -- Erythrocytes; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2019.04.008
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Currently, more and more concerns are related to oxidative stress appearing in cells as a result of xenobiotics action. It has been found that selected brominated flame retardants (BFRs) can cause reactive oxygen species (ROS) induction at environmental concentrations. Excessive ROS induction can contribute to the redox imbalance in the cell. Therefore, the aim of our work was to evaluate the effect of selected BFRs on the activity of antioxidant enzymes, i.e. superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and the level of reduced glutathione (GSH) in human erythrocytes. Erythrocytes were incubated with tetrabromobisphenol A (TBBPA), tetrabromobisphenol S (TBBPS), 2, 4-dibromophenol (2, 4-DBP), 2, 4, 6-tribromophenol (2, 4, 6-TBP) and pentabromophenol (PBP) in the concentration ranging from 1 to 100 μg/ml. This study has shown that the BFRs studied disturbed redox balance in human erythrocytes. TBBPA caused more significant decrease in antioxidant enzymes activities than other compounds examined. Among bromophenols studied, 2, 4-DBP most strongly affected antioxidant system, which indicated that the number of bromine atoms in the molecule did not significantly affect the pro-oxidative properties of the BFRs examined. Highlights: Effect of BFRs on selected oxidative stress parameters in erythrocytes were studied. BFRs induced oxidative stress by decreasing SOD, CAT, GSH-Px activity and GSH level. TBBPA induced stronger prooxidative alterations in human erythrocytes than TBBPS. Among bromophenols 2, 4-DBP induced the highest changes in antioxidative system.