High-resolution colorimetric detection of lipase activity based on enzyme-controlled reshaping of gold nanorods. Issue 17 (5th April 2019)

• Record Type: Journal Article
• Authors: Zhang, Hao; Wu, Shengnan; Zhang, Linghua; Jiang, Ling; Huo, Fengwei; Tian, Danbi
• Is Part Of: Analytical methods. Volume 11:Issue 17(2019)
• Page Start: 2286
• Page End: 2291
• Publication Date: 2019-04-05
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/c9ay00410f
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : The sensing mechanism of a high resolution colorimetric assay for the visual readout of lipase activity based on AuNR reshaping. Abstract : We developed a novel enzyme-controlling colorimetric assay to measure lipase activity. The method relied on enzymatic reaction-assisted gold deposition on gold nanorods (AuNRs) to generate a significant color change, which was strongly dependent on lipase activity. The H2 O2 produced by autoxidation of the ethylene oxide subunits in Tween 80 reduced AuCl4 − ions to Au atoms, which were coated onto of the surface of the AuNRs. In addition, lipase catalyzed the hydrolysis of the carboxyl ester bond in Tween 80, which controlled the rate of reshaping of AuNRs and tailored the localized surface plasmon resonance of the AuNRs. A linear response ranging from 0 to 4.5 mg mL −1 and a detection limit of 0.017 mg mL −1 for lipase were obtained. The methodology provided a sensitive, convenient, and rapid assay to monitor enzyme activities by judging different colors using both naked-eye detection and colorimetric instruments.