Ultrasensitive detection of lead ions based on a DNA-labelled DNAzyme sensor. Issue 2 (4th December 2014)

• Record Type: Journal Article
• Authors: Zhu, Yingyue; Deng, Daqing; Xu, Liguang; Zhu, Yibo; Wang, Limei; Qi, Bin; Xu, Chuanlai
• Is Part Of: Analytical methods. Volume 7:Issue 2(2015)
• Page Start: 662
• Page End: 666
• Publication Date: 2014-12-04
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/c4ay02654c
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : Here, we report a facile approach for highly sensitive and selective detection of aqueous lead ions that uses a real-time quantitative polymerase chain reaction technology and a lead-dependent DNAzyme, termed GR-5. Abstract : Here, we report a facile approach for highly sensitive and selective detection of aqueous lead ions that uses a real-time quantitative polymerase chain reaction technology and a lead-dependent DNAzyme, termed GR-5. In this method, the substrate DNA is cleaved at the site of the adenosine ribonucleotide by GR-5 DNAzyme in the presence of lead ions, resulting in a decrease in template DNA available for PCR and a consequent change in signal detection (cycle threshold (Ct) value). This novel approach takes advantage of the exponential amplification of PCR and the specific recognition of the GR-5 lead-dependent DNAzyme to provide Pb 2+ -specific detection with an excellent linear relationship between Ct value and Pb 2+ concentration within a range of 1–500 nM. The correlation coefficient of the standard curve was 0.9898, and the limit of detection was 0.7 nM. Moreover, this sensor showed good selectivity for Pb 2+ ions over other metal ions.