Probing unnatural amino acid integration into enhanced green fluorescent protein by genetic code expansion with a high-throughput screening platform. Issue 1 (December 2016)

• Record Type: Journal Article
• Authors: Wandrey, Georg; Wurzel, Joel; Hoffmann, Kyra; Ladner, Tobias; Büchs, Jochen; Meinel, Lorenz; Lühmann, Tessa
• Is Part Of: Journal of biological engineering. Volume 10:Issue 1(2016)
• Page Start: 1
• Page End: 13
• Publication Date: 2016-12
• Subjects: Amber codon suppression -- Online monitoring system -- High-throughput screening -- Unnatural amino acid -- Bio-orthogonal chemistry -- Protein engineering; Bioengineering -- Periodicals; 660.6
• DOI: 10.1186/s13036-016-0031-6
• Languages: English
• ISSNs: 1754-1611

Abstract

Abstract Background Genetic code expansion has developed into an elegant tool to incorporate unnatural amino acids (uAA) at predefined sites in the protein backbone in response to an amber codon. However, recombinant production and yield of uAA comprising proteins are challenged due to the additional translation machinery required for uAA incorporation. Results We developed a microtiter plate-based high-throughput monitoring system (HTMS) to study and optimize uAA integration in the model protein enhanced green fluorescence protein (eGFP). Two uAA, propargyl-L-lysine (Plk) and (S)-2-amino-6-((2-azidoethoxy) carbonylamino) hexanoic acid (Alk), were incorporated at the same site into eGFP co-expressing the native PylRS/tRNAPyl CUA pair originating fromMethanosarcina barkeri inE. coli . The site-specific uAA functionalization was confirmed by LC-MS/MS analysis. uAA-eGFP production and biomass growth in parallelizedE. coli cultivations was correlated to (i) uAA concentration and the (ii) time of uAA addition to the expression medium as well as to induction parameters including the (iii) time and (iv) amount of IPTG supplementation. The online measurements of the HTMS were consolidated by end point-detection using standard enzyme-linked immunosorbent procedures. Conclusion The developed HTMS is powerful tool for parallelized and rapid screening. In light of uAA integration, future applications may include parallelized screening of different PylRS/tRNAPyl CUA pairs as well as further optimization of culture conditions.