Comprehensive analysis of different adhesives in aerobiological sampling using optical microscopy and high-throughput DNA sequencing. (15th June 2019)

• Record Type: Journal Article
• Authors: Rojo, Jesús; Núñez, Andrés; Lara, Beatriz; Sánchez-Parra, Beatriz; Moreno, Diego A.; Pérez-Badia, Rosa
• Is Part Of: Journal of environmental management. Volume 240(2019)
• Page Start: 441
• Page End: 450
• Publication Date: 2019-06-15
• Subjects: Aerobiology -- Adhesive -- Morphological analysis -- High-throughput DNA sequencing -- Pollen; Environmental policy -- Periodicals; Environmental management -- Periodicals; Environment -- Periodicals; Ecology -- Periodicals; 363.705
• DOI: 10.1016/j.jenvman.2019.03.116
• Languages: English
• ISSNs: 0301-4797

Abstract

Abstract: The standardization and unification of the procedures to analyze and quantify the airborne pollen concentrations are very important topics. In this work, the effectiveness of the two most used adhesives in aerobiological sampling, silicone prepared with cyclohexane solvent (Silicone) and petroleum jelly (Vaseline), was compared under outdoor conditions. This comparison was carried out using the traditional method based on the identification and quantification by optical microscopy (OM) of the airborne pollen and the novel methodology by high-throughput sequencing analysis (HTS). Globally, the results from both methods of analysis (OM and HTS) showed a good agreement between the two adhesives tested regarding the abundance of the main pollen types present in the samples: Cupressaceae, Olea, Poaceae, Platanus, Quercus . We concluded that the results from both adhesives are comparable data. Furthermore, the comparisons between methodologies, OM vs. HTS, showed that both techniques can accurately identify the most abundant pollen types in the atmosphere for the studied periods, with a good agreement of their relative abundances especially when the airborne pollen diversity is low but showing some divergences as the number of pollen types increases. Graphical abstract: Image 1 Highlights: Two adhesives were compared in aerobiological pollen sampling under outdoor conditions. Both adhesives, Silicone vs. Vaseline, showed a good agreement using optical microscopy. Only Pinus pollen captured in Silicone was higher than those registered in Vaseline. The same results were observed using high-throughput DNA sequencing. Vaseline can be an appropriate substitute for Silicone and vice versa.