Ceria‐Nanoparticles‐Based Microfluidic Nanobiochip Electrochemical Sensor for the Detection of Ochratoxin‐A. Issue 17 (1st May 2019)

• Record Type: Journal Article
• Authors: Dhiman, Tarun K.; Lakshmi, GBVS; Roychoudhury, Appan; Jha, Sandeep K.; Solanki, Pratima R.
• Is Part Of: ChemistrySelect. Volume 4:Issue 17(2019)
• Page Start: 4867
• Page End: 4873
• Publication Date: 2019-05-01
• Subjects: Cerium Oxide, Electrochemistry, Microfluidics, Mycotoxins, Ochratoxin-A; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/slct.201803752
• Languages: English
• ISSNs: 2365-6549

Abstract

Abstract: Ochratoxin‐A (OTA) is a fungal mycotoxin that is present in most of the food stuff, and shows adverse effects on human beings. Therefore, the rapid and accurate detection of OTA is necessary. Here, ceria nanoparticles based microfluidic nanobiochip was fabricated for the electrochemical detection of OTA. Ceria nanoparticles were synthesized using a chemical co‐precipitation method and characterized by various techniques. Raman spectroscopy confirmed the formation of fluorite cubic structure. Transmission electron microscopy (TEM) exhibited the formation of very small size (4‐5 nm) with nearly spherical shape nanoparticles. Microfluidic nanobiochip was fabricated using 300 μm microfluidic channel using polydimethylsiloxane (PDMS). Simultaneously, indium tin oxide (ITO) coated Corning glass was used for the fabrication of immunoelectrodes platform using antibodies (anti‐OTA) for the OTA detection. The detection range was achieved from 350 pg ml −1 to 10 ng ml −1 with sensitivity of 7.5 μA ng −1 mL cm −2, without any interference effect using differential pulse voltammetry. Abstract : In the present paper, a microfluidic electrochemical immunosensor (nanobiochip) for the detection Ochratoxin‐A (OTA) was fabricated using ceria nanoparticles (CeO2 Nps) coated Indium Tin Oxide (ITO) electrodes. The CeO2 Nps were synthesized by chemical co‐precipitation method and characterized with various methods such as raman spectroscopy, fourier transform infrared (FTIR) spectroscopy, field emission scanning electron microscope (FESEM), transmission electron microscope (TEM), cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The immunosensor is used for the detection of OTA and the sensitivity was obtained to be 7.5 μA ng −1 ml cm −2 with lower detection of 350 pg mL −1 .