Indirect Competitive Aptamer-Based Enzyme-Linked Immunosorbent Assay (apt-ELISA) for the Specific and Sensitive Detection of Isocarbophos Residues. (13th August 2019)

• Record Type: Journal Article
• Authors: Xiang, Li; Wu, Huanle; Cui, Zhaoxing; Tang, Jianshe
• Is Part Of: Analytical letters. Volume 52:Number 12(2019)
• Page Start: 1966
• Page End: 1975
• Publication Date: 2019-08-13
• Subjects: Absorbance analysis -- aptamer-based enzyme-linked immunosorbent assay (apt-ELISA) -- isocarbophos -- indirect competitive assay; Chemistry, Analytic -- Periodicals; Chemistry, Analytic -- Abstracts; 543
• DOI: 10.1080/00032719.2019.1587446
• Languages: English
• ISSNs: 0003-2719

Abstract

Abstract: Environmental contamination from pesticide residues is a worldwide public health concern and has attracted increasing attention from researchers over the past several years. In this study, an aptamer-based enzyme-linked immunosorbent assay (apt-ELISA) using enzyme signal amplification has been developed to determine isocarbophos residues. The limit of detection for the apt-ELISA was 0.05 ng/mL for isocarbophos, and the sensitivity was better than that obtained using the traditional ELISA approach. The average recovery of the apt-ELISA ranged from 84 to 97%, and the coefficient of variation ranged from 4.6 to 12.1% when determining the isocarbophos residues in various water samples. The results demonstrate that the proposed approach can be used for routine screening of isocarbophos residues.