A simultaneous triplex TaqMan real-time PCR approach for authentication of caprine and bovine meat, milk and cheese. (August 2019)

• Record Type: Journal Article
• Authors: Guo, Liang; Ya, Mei; Hai, Xiao; Guo, Yuan-Sheng; Li, Chun-Dong; Xu, Wei-Liang; Liao, Cheng-Song; Feng, Wei; Cai, Qiang
• Is Part Of: International dairy journal. Volume 95(2019)
• Page Start: 58
• Page End: 64
• Publication Date: 2019-08
• Subjects: Dairying -- Periodicals; Industrie laitière -- Périodiques; Electronic journals; 338.1762142
• DOI: 10.1016/j.idairyj.2019.03.004
• Languages: English
• ISSNs: 0958-6946

Abstract

Abstract: Goat foodstuffs are considered as healthy foods with high nutritional value. This study demonstrated the development and validation of a triplex real-time PCR on the basis of species-specific and species-conservative Taq Man probes for the simultaneous identification of caprine and bovine DNA in meats, milk and cheeses with a prerequisite designed endogenous control. In this research, caprine and bovine meat, milk and cheese were specifically identified via developed primers and probes, and the limits of detection of this methodology were 0.005 and 0.01 ng DNA of milk and cheese from goat, and 0.01 and 0.05 ng DNA of milk and cheese from cow. Taken together, this approach was elaborated to address dairy adulteration issues to eliminate the fraud of economically motivated goat milk and cheese adulteration by adding cow milk.