Protein adsorption characteristics of nanoparticle-assembled hollow microspheres of hydroxyapatite and their composites with PLLA microporous membranes. Issue 4 (April 2019)

• Record Type: Journal Article
• Authors: Tanaka, Takaaki; Takai, Yoshihiko; Nagase, Akifumi; Teraguchi, Kazuki; Minbu, Hiromi; Ochiai, Akihito; Kimura, Isao; Taniguchi, Masayuki
• Is Part Of: Heliyon. Volume 5:Issue 4(2019)
• Publication Date: 2019-04
• Subjects: Materials science -- Materials chemistry -- Physical chemistry; Research -- Periodicals; Medical sciences -- Periodicals; Natural history -- Periodicals; Social sciences -- Periodicals; Earth sciences -- Periodicals; Physical sciences -- Periodicals; 507.2
• DOI: 10.1016/j.heliyon.2019.e01490
• Languages: English
• ISSNs: 2405-8440

Abstract

Abstract: Nanoparticle-assembled hydroxyapatite (HA) hollow microspheres have a high surface area and are convenient to handle, owing to their characteristic structure. In this study we characterized the protein adsorption of HA hollow microspheres prepared from CaCl2 and K2 HPO4 by a water-in-oil-in-water (W/O/W) emulsion method assisted by two surfactants: Span 80 and Tween 20. The HA hollow microspheres adsorbed bovine serum albumin, bovine γ-globulin, equine skeletal muscle myoglobin, and chicken egg white lysozyme in 10 mM sodium phosphate buffer (pH 6.8) in a Langmuir-type adsorption and desorbed the proteins in 800 mM sodium phosphate buffer (pH 6.8). The maximum adsorbed amounts of the HA hollow microspheres were 7.5–9.0 times higher than those of the microrods with a similar size range. The composite membranes of the HA microspheres and the poly(l -lactic acid) (PLLA) microporous membranes exhibited a high adsorption capacity for γ-globulin.