Diagnosis of epidermodysplasia verruciformis: Two cases highlighting the role of direct HPV L1 gene sequencing. (28th March 2019)

• Record Type: Journal Article
• Authors: Bushara, Omar; Miller, Daniel; Giubellino, Alessio; Schomaker, Matthew; Thyagarajan, Bharat; Nelson, Andrew C.
• Is Part Of: Journal of cutaneous pathology. Volume 46:Number 6(2019)
• Page Start: 436
• Page End: 441
• Publication Date: 2019-03-28
• Subjects: DNA sequence analysis -- epidermodysplasia verruciformis -- genotype -- papillomavirus infections -- polymerase chain reaction; Skin -- Diseases -- Periodicals; Dermatology -- Periodicals; 616
• DOI: 10.1111/cup.13444
• Languages: English
• ISSNs: 0303-6987

Abstract

Abstract : Epidermodysplasia verruciformis (EV) is a rare skin disease characterized by the development of multiple flat warts with the potential for malignant transformation. Patients are susceptible to human papillomavirus (HPV) infection that develops in a background of either a genetic or acquired immunodeficiency predisposing patients to infection with specific HPV types that are ubiquitous but generally non‐pathogenic in healthy individuals. There is no standard clinical methodology for determining the causative HPV from patients with suspected EV. Here, we report the diagnostic workup of two EV cases and describe the use of L1 gene Sanger sequencing as a specific method to accurately identify the causative HPV genotype and confirm the diagnosis of EV.