Defining mesenchymal stromal cell (MSC)-derived small extracellular vesicles for therapeutic applications. Issue 1 (1st January 2019)

• Record Type: Journal Article
• Authors: Witwer, Kenneth W.; Van Balkom, Bas W.M.; Bruno, Stefania; Choo, Andre; Dominici, Massimo; Gimona, Mario; Hill, Andrew F.; De Kleijn, Dominique; Koh, Mickey; Lai, Ruenn Chai; Mitsialis, S. Alex; Ortiz, Luis A.; Rohde, Eva; Asada, Takashi; Toh, Wei Seong; Weiss, Daniel J.; Zheng, Lei; Giebel, Bernd; Lim, Sai Kiang
• Is Part Of: Journal of extracellular vesicles. Volume 8:Issue 1(2019)
• Publication Date: 2019-01-01
• Subjects: Please check whether the inserted Keywords are correct -- MSC -- sEV -- Therapeutics -- Definition; Cells -- Mechanical properties -- Periodicals; Transport Vesicles; Cells -- Mechanical properties; Periodicals; Fulltext; Internet Resources; Periodicals; Electronic journals; Periodicals; 571.63
• DOI: 10.1080/20013078.2019.1609206
• Languages: English
• ISSNs: 2001-3078

Abstract

ABSTRACT: Small extracellular vesicles (sEVs) from mesenchymal stromal/stem cells (MSCs) are transiting rapidly towards clinical applications. However, discrepancies and controversies about the biology, functions, and potency of MSC-sEVs have arisen due to several factors: the diversity of MSCs and their preparation; various methods of sEV production and separation; a lack of standardized quality assurance assays; and limited reproducibility of in vitro and in vivo functional assays. To address these issues, members of four societies (SOCRATES, ISEV, ISCT and ISBT) propose specific harmonization criteria for MSC-sEVs to facilitate data sharing and comparison, which should help to advance the field towards clinical applications. Specifically, MSC-sEVs should be defined by quantifiable metrics to identify the cellular origin of the sEVs in a preparation, presence of lipid-membrane vesicles, and the degree of physical and biochemical integrity of the vesicles. For practical purposes, new MSC-sEV preparations might also be measured against a well-characterized MSC-sEV biological reference. The ultimate goal of developing these metrics is to map aspects of MSC-sEV biology and therapeutic potency onto quantifiable features of each preparation.