A protein-mRNA feedback exists in miR-21-associated E-selectin expression. (1st May 2019)

• Record Type: Journal Article
• Authors: Tang, Siyuan; Liu, Bailong; Liu, Jiaqi; Wang, Jian; Wang, Ya
• Is Part Of: International journal of radiation biology. Volume 95:Number 5(2019)
• Page Start: 580
• Page End: 584
• Publication Date: 2019-05-01
• Subjects: miR-21 -- radiation -- lung tumorigenesis -- immune response -- Sele; Radiation -- Physiological effect -- Periodicals; Radiobiology -- Periodicals; 571.45
• DOI: 10.1080/09553002.2019.1564082
• Languages: English
• ISSNs: 0955-3002

Abstract

Abstract: Purpose: To study whether miR-21, an oncogene associated with lung tumorigenesis, affects immune response. Material and methods: Cancer immune-related 786 mRNA expression was compared in lung tissue from wild- type and miR-21 knock-in mice using NanoString technology. The significantly changed genes were verified using real-time PCR. E-Selectin (Sele) was subsequently identified for further examination using immunohistochemistry (IHC) and Western blot in the same lung tissue. The mouse Sele 3'untranslated region (3'-UTR) was searched to identify a miR-21 matching sequence. The Sele level in miR-21 mimic transfected mouse lung bronchial epithelial (LBE) cells was examined. Results: We unexpectedly found that the Sele mRNA level significantly increased but the protein level significantly decreased in the lung tissue of miR-21 knock-in mice compared to the mRNA/protein levels in the lung tissue of wild- type mice. The mouse Sele 3'-UTR contains the key sequence that can be targeted by miR-21. The Sele levels decreased in mouse LBE cells after miR-21 mimic transfection. Conclusion: Sele is a potential miR-21 target. The opposing Sele levels at mRNA and protein suggest a feedback-regulation from protein to mRNA. The feedback-regulation in miR-21-suppressed gene expression indicates that we should carefully evaluate any data from mRNA array since they may not reflect real protein expression status.