Dynamic changes in miR-124 levels in patients with acute cerebral infarction. (3rd July 2019)

• Record Type: Journal Article
• Authors: Sun, Mei; Hou, Xiaoxia; Ren, Guang; Zhang, Yangyang; Cheng, Hong
• Is Part Of: International journal of neuroscience. Volume 129:Number 7(2019)
• Page Start: 649
• Page End: 653
• Publication Date: 2019-07-03
• Subjects: Acute cerebral infarction -- miR-124 -- iASPP -- apoptosis; Nervous system -- Periodicals; 612.805
• DOI: 10.1080/00207454.2018.1513931
• Languages: English
• ISSNs: 0020-7454

Abstract

Abstract: Objective: To investigate the changes in serum miR-124 levels in patients with acute cerebral infarction (ACI) and elucidate the underlying mechanism by a dynamic monitor. Methods: Fifty-four patients with ACI and 51 healthy controls were included in our study. Baseline characteristics and blood samples were collected for further analysis. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure the serum miR-124 levels. The dual-luciferase reporter assay was used to evaluate the effect of miR-124 on iASPP, a protein that inhibits apoptosis stimulating proteins in the p53 family. Results: Compared with normal controls, the miR-124 levels in the ACI group rapidly decreased at phase 1 (within 24 h after ischemia) ( p < 0.001) and then gradually increased at phase 2 (48 ∼ 72 h after ischemia) ( p < 0.001) and phase 3 (the 7th day after ischemia) ( p < 0.001). The dual-luciferase reporter assay showed that miR-124 down-regulates iASPP expression in 293T cells. Conclusion: The miR-124 levels are down-regulated in ACI patients. The dynamic changes of miR-124 might provide a possible method for the detection of ischemic stroke. Highlights: The difference in miR-124 expression levels between ACI patients and normal controls. Dynamic changes of miR-124 expression levels in ACI patients. The down-regulation of miR-124 upon iASPP expression.