Detecting total toxicity in water using a mediated biosensor system with flow injection. (November 2015)

• Record Type: Journal Article
• Authors: Yong, Daming; Liu, Changyu; Zhu, Chengzhou; Yu, Dengbin; Liu, Ling; Zhai, Junfeng; Dong, Shaojun
• Is Part Of: Chemosphere. Volume 139(2015)
• Page Start: 109
• Page End: 116
• Publication Date: 2015-11
• Subjects: Mediated biosensor -- Immobilized microorganism -- Total toxicity -- Flow injection mode; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2015.05.031
• Languages: English
• ISSNs: 0045-6535

Abstract

Highlights: This paper provides a biosensor for toxicity monitoring. This method is a simple, rapid and low cost means. This constructed biosensor system will easier to be applied and commercialized. Abstract: A novel total toxicity detection method based on a mediated biosensor system with flow injection (MB-FI) was developed to rapidly and reliably detect respiration inhibitors (i.e., As2 O3, KCN, salicylic acid (SA), 2, 4-dintirophenol (DNP)) in water. The mediated biosensor toxicity assessment using microorganisms immobilized in calcium alginate filaments can greatly simplify the testing process and save time. In the MB-FI system, ferricyanide together with a respiration inhibitor was injected into the bioreactor, inhibiting the respiration of the immobilized microorganisms. The degree of inhibition was measured by determining the ferrocyanide generated in the effluent, expressed as the 50% inhibition concentration (IC50). The IC50 values for the four respiration inhibitors obtained using this method were comparable to those obtained using the classic method, confirming that this approach is an alternative alert method. More importantly, this constructed biosensor system with flow injection will facilitate the application and commercialization of this toxicity monitoring technology.