Arginine mimetic appended peptide-based probes for fluorescence turn-on detection of 14-3-3 proteins. Issue 17 (12th April 2019)

• Record Type: Journal Article
• Authors: Maity, Debabrata; Gigante, Alba; Sánchez-Murcia, Pedro A.; Sijbesma, Eline; Li, Mao; Bier, David; Mosel, Stefanie; Knauer, Shirley; Ottmann, Christian; Schmuck, Carsten
• Is Part Of: Organic & biomolecular chemistry. Volume 17:Issue 17(2019)
• Page Start: 4359
• Page End: 4363
• Publication Date: 2019-04-12
• Subjects: Chemistry, Organic -- Periodicals; Bioorganic chemistry -- Periodicals; Chemistry, Physical organic -- Periodicals; 547
• DOI: 10.1039/c9ob00620f
• Languages: English
• ISSNs: 1477-0520

Abstract

Abstract : Development of fluorescence markers for the 14-3-3 adapter protein class. Abstract : 14-3-3 proteins are adaptor elements in intracellular signaling pathways. Recently, this protein family has been identified as a relevant therapeutic target involved in many human diseases. Therefore, identification of 14-3-3 proteins in biological systems is very important. Two cationic peptide-based probes are reported for the fluorescence detection of 14-3-3 proteins at physiological pH. The design of these probes consists of two symmetric peptidic arms equipped with a guanidiniocarbonyl pyrrole moiety (an arginine mimetic aka GCP), and an environment-sensitive amino-naphthalimide fluorophore as a third arm. These peptide sequences also contain lysine and phenylalanine/tryptophan amino acids for additional charge–charge and hydrophobic interactions. Both probes show high affinity and sensitivity for the 14-3-3 family, as well as good selectivity against other relevant biological proteins and ions.