Identification of acetyltransferase genes (HAT1 and KAT8) regulating HBV replication by RNAi screening. Issue 1 (December 2015)

• Record Type: Journal Article
• Authors: Wang, Hui; Liu, KeHui; Fang, Bernard; Wu, HaiQing; Li, FengDi; Xiang, XiaoGang; Tang, WeiLiang; Zhao, GangDe; Lin, LanYi; Bao, Shisan; Xie, Qing
• Is Part Of: Cell & bioscience. Volume 5:Issue 1(2015)
• Page Start: 1
• Page End: 9
• Publication Date: 2015-12
• Subjects: KAT8, HAT1, HBV replication -- pgRNA -- RNAi screening; Biology -- Periodicals; Life sciences -- Periodicals; Cytology -- Periodicals; 571.6
• DOI: 10.1186/s13578-015-0059-1
• Languages: English
• ISSNs: 2045-3701

Abstract

Abstract Background The initiation of hepatitis B virus (HBV) replication involves the formation of covalently closed circular DNA (cccDNA) and its transcription into pregenomic RNA (pgRNA) in hepatocyte nuclei. The regulatory mechanism of HBV replication by acetyltransferase is thus far not well understood, but human acetyltransferase has been reported as being involved in the regulation of HBV replication. Results Depletion ofKAT8 orHAT1 via RNA interference (RNAi) markedly down-regulated HBV-DNA and pgRNA levels in HepG2.2.15 cells, withKAT8 knockdown reducing both HBsAg and HBeAg more thanHAT1 knockdown. Consistent with these observations, HBV replication regulators hepatocyte nuclear factor-4-α (HNF4α) and peroxisome proliferator-activated receptor gamma coactivator- (PPARGC-) 1-α were decreased following knockdown ofHAT1 orKAT8 . Conclusions These data suggest thatKAT8 orHAT1 regulate HBV replication and may be potential drug targets of anti-HBV therapy.