A membrane-activatable near-infrared fluorescent probe with ultra-photostability for mitochondrial membrane potentials. Issue 12 (4th November 2015)

• Record Type: Journal Article
• Authors: Ren, Wei; Ji, Ao; Karmach, Omran; Carter, David G.; Martins-Green, Manuela M.; Ai, Hui-wang
• Is Part Of: Analyst. Volume 141:Issue 12(2016)
• Page Start: 3679
• Page End: 3685
• Publication Date: 2015-11-04
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c5an01860a
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : Dark for light : A fluorescence quencher was turned into a near-infrared probe for mitochondrial membrane potential in living cells and mice. Abstract : Mitochondrial membrane potential (MMP) is a frequently used indicator for mitochondrial function. Herein, we report a photostable near-infrared (NIR) fluorescent dye for monitoring MMP. This new probe, named NIMAP, is non-fluorescent in aqueous solution and can be activated by cell membranes, providing high fluorescence contrast and low background fluorescence. NIMAP has been validated for monitoring MMP in living mammalian cells and in mice. Due to the large fluorescence response, low fluorescence background, high photostability, and excellent tissue penetration resulting from red-shifted excitation and emission in the "optical window" above 600 nm, broad applications of this new probe are expected.