Comparison of American mink embryonic stem and induced pluripotent stem cell transcriptomes. (December 2015)

• Record Type: Journal Article
• Authors: Menzorov, Aleksei; Matveeva, Natalia; Markakis, Marios; Fishman, Venyamin; Christensen, Knud; Khabarova, Anna; Pristyazhnyuk, Inna; Kizilova, Elena; Cirera, Susanna; Anistoroaei, Razvan; Serov, Oleg
• Is Part Of: BMC genomics. Volume 16:Number 13(2015)
• Page Start: 1
• Page End: 12
• Publication Date: 2015-12
• Subjects: pluripotency -- reprogramming -- American mink -- iPS cells -- ES cells -- Nanog -- transcriptome; Genomes -- Periodicals; Gene mapping -- Periodicals; Genomics -- Periodicals; Base Sequence -- Periodicals; Chromosome Mapping -- Periodicals; Genetic Techniques -- Periodicals; Sequence Analysis, DNA -- Periodicals; 572.8605
• DOI: 10.1186/1471-2164-16-S13-S6
• Languages: English
• ISSNs: 1471-2164

Abstract

Abstract Background Recently fibroblasts of many mammalian species have been reprogrammed to pluripotent state using overexpression of several transcription factors. This technology allows production of induced pluripotent stem (iPS) cells with properties similar to embryonic stem (ES) cells. The completeness of reprogramming process is well studied in such species as mouse and human but there is not enough data on other species. We produced American mink (Neovison vison ) ES and iPS cells and compared these cells using transcriptome analysis. Results We report the generation of 10 mink ES and 22 iPS cell lines. The majority of the analyzed cell lines had normal diploid chromosome number. The only ES cell line with XX chromosome set had both X-chromosomes in active state that is characteristic of pluripotent cells. The pluripotency of ES and iPS cell lines was confirmed by formation of teratomas with cell types representing all three germ layers. Transcriptome analysis of mink embryonic fibroblasts (EF), two ES and two iPS cell lines allowed us to identify 11831 assembled contigs which were annotated. These led to a number of 6891 unique genes. Of these 3201 were differentially expressed between mink EF and ES cells. We analyzed expression levels of these genes in iPS cell lines. This allowed us to show that 80% of genes were correctly reprogrammed in iPS cells, whereas approximately 6% had an intermediate expression pattern, about 7% were not reprogrammed and about 5% had a "novel" expression pattern. We observed expression of pluripotency marker genes such asOct4, Sox2 andRex1 in ES and iPS cell lines with notable exception ofNanog . Conclusions We had produced and characterized American mink ES and iPS cells. These cells were pluripotent by a number of criteria and iPS cells exhibited effective reprogramming. Interestingly, we had showed lack ofNanog expression and consider it as a species-specific feature.