Haemodiafiltration elicits less platelet activation compared to haemodialysis. Issue 1 (December 2016)

• Record Type: Journal Article
• Authors: Becs, Gergely; Hudák, Renáta; Fejes, Zsolt; Debreceni, Ildikó; Bhattoa, Harjit; Balla, József; Kappelmayer, János
• Is Part Of: BMC nephrology. Volume 17:Issue 1(2016)
• Page Start: 1
• Page End: 10
• Publication Date: 2016-12
• Subjects: ESRD -- Haemodiafiltration -- Haemodialysis -- Monocyte-platelet aggregate -- P-selectin; Kidneys -- Diseases -- Periodicals; 616.61005
• DOI: 10.1186/s12882-016-0364-x
• Languages: English
• ISSNs: 1471-2369

Abstract

Abstract Background Mortality in patients with end-stage renal disorders is often a consequence of cardiovascular complications. Renal replacement therapies may contribute to this morbidity by promoting cellular activation. In renal failure patients peripheral blood samples were investigated for platelet and endothelial cell activation markers to compare the effects of haemodiafiltration (HDF) and haemodialysis (HD). Methods Overall 28 patients were included in the study. Platelet P-selectin and leukocyte - platelet heterotypic aggregates were studied by flow cytometry. Soluble P- and E-selectin values were determined by ELISA, while von Willebrand factor (vWF) antigen levels were measured by immunoturbidimetry. Statistical analysis was done by the SPSS v22 software. Results Platelet surface P-selectin was below 3.0 % in healthy controls, but it was higher during the dialysis after 4 h, 8 % and 14.3 % in HDF and HD, respectively. Monocyte-platelet heterotypic aggregates were significantly elevated after 4 h in both treatments, up to 69.2 % in HDF and to 82.9 % in HD. Soluble P-selectin levels were also significantly elevated by the end of both treatment procedures (p < 0.001), vWF antigen values, however, showed elevation only during HD treatment. Conclusions The attenuated platelet activating effects of HDF compared to HD may contribute to a less unfavourable vascular effect in this treatment modality.