Modulation of excitatory synaptic transmission in rat hippocampal CA3 neurons by triphenyltin, an environmental pollutant. (February 2015)

• Record Type: Journal Article
• Authors: Wakita, Masahito; Oyama, Yasuo; Takase, Yuko; Akaike, Norio
• Is Part Of: Chemosphere. Volume 120(2015)
• Page Start: 598
• Page End: 607
• Publication Date: 2015-02
• Subjects: Triphenyltin -- Synaptic transmission -- Glutamate -- Calcium; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2014.09.073
• Languages: English
• ISSNs: 0045-6535

Abstract

Highlights: Adverse effects of triphenyltin are still a significant concern. Triphenyltin facilitated glutamatergic synaptic transmission of neurons in the brain. This facilitation was due to the effect of triphenyltin on intracellular Ca 2+ stores. Triphenyltin may affect long-term potentiation at nanomolar concentrations. Abstract: Triphenyltin (TPT) is an organometallic compound that poses a known environmental hazard to some fish and mollusks, as well as mammals. However, its neurotoxic mechanisms in the mammalian brain are still unclear. Thus, we have investigated mechanisms through which TPT modulates glutamatergic synaptic transmission, including spontaneous, miniature, and evoked excitatory postsynaptic currents (sEPSCs, mEPSCs, and eEPSCs respectively), in a rat hippocampal CA3 'synaptic-bouton' preparation. TPT, at environmentally relevant concentrations (30 nM to 1 μM), significantly increased the frequency of sEPSCs and mEPSCs in a concentration-dependent manner, without affecting the currents' amplitudes. The facilitatory effects of TPT on mEPSC frequency were seen even in a Ca 2+ -free external solution containing tetrodotoxin. These effects were further prolonged by adding caffeine, which releases Ca 2+ from intracellular Ca 2+ storage sites. In glutamatergic eEPSCs evoked by paired-pulse stimuli, TPT at concentrations greater than or equal to 100 nM markedly increased the current amplitude by the first pulse and decreased failure rate and pair-pulse ratio. On the other hand, both voltage-dependent Na + and Ca 2+ channels were unaffected by submicromolar concentrations of TPT. Overall, these results suggest that TPT, at environmentally relevant concentrations, affects presynaptic transmitter release machinery by directly modulating Ca 2+ storage. Further, findings of this study imply that excitotoxic mechanisms may underlie TPT-induced neuronal damage.