Dose- and time-dependent expression of aryl hydrocarbon receptor (AhR) and aryl hydrocarbon receptor nuclear translocator (ARNT) in PCB-, B[a]P-, and TBT-exposed intertidal copepod Tigriopus japonicus. (February 2015)

• Record Type: Journal Article
• Authors: Kim, Bo-Mi; Rhee, Jae-Sung; Hwang, Un-Ki; Seo, Jung Soo; Shin, Kyung-Hoon; Lee, Jae-Seong
• Is Part Of: Chemosphere. Volume 120(2015)
• Page Start: 398
• Page End: 406
• Publication Date: 2015-02
• Subjects: Copepod -- Tigriopus japonicus -- AhR -- ARNT -- Polychlorinated biphenyl -- Benzo[a]pyrene; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2014.07.099
• Languages: English
• ISSNs: 0045-6535

Abstract

Highlights: Cloning of Tj - AhR and Tj - ARNT genes from the copepod Tigriopus japonicus. Dose- and time-dependent expression of Tj - AhR and Tj - ARNT genes against PCB-, B[ a ]P-, and TBT exposure. Potential role of Tj-AhR and Tj-ARNT in the putative AhR-CYP signaling pathway. Abstract: The aryl hydrocarbon receptor ( AhR ) and aryl hydrocarbon nuclear translocator ( ARNT ) genes from the copepod Tigriopus japonicus ( Tj ) were cloned to examine their potential functions in the invertebrate putative AhR-CYP signaling pathway. The amino acid sequences encoded by the Tj-AhR and Tj-ARNT genes showed high similarity to homologs of Daphnia and Drosophila, ranging from 68% and 70% similarity for the AhR genes to 56% for the ARNT genes. To determine whether Tj-AhR and Tj-ARNT are modulated by environmental pollutants, transcriptional expression of Tj-AhR and Tj-ARNT was analyzed in response to exposure to five concentrations of polychlorinated biphenyl (PCB 126) (control, 10, 50, 100, 500 μg L −1 ), benzo[ a ]pyrene (B[ a ]P) (control, 5, 10, 50, 100 μg L −1 ), and tributyltin (TBT) (control, 1, 5, 10, 20 μg L −1 ) 24 h after exposure. A time-course experiment (0, 3, 6, 12, 24 h) was performed to analyze mRNA expression patterns after exposure to PCB, B[ a ]P, and TBT. T. japonicus exhibited dose-dependent and time-dependent upregulation of Tj-AhR and Tj-ARNT in response to pollutant exposure, and the degree of expression was dependent on the pollutant, suggesting that pollutants such as PCB, B[ a ]P, and TBT modulate expression of Tj-AhR and Tj-ARNT genes in the putative AhR-CYP signaling pathway.