Rapid, automated detection of stem canker symptoms in woody perennials using artificial neural network analysis. Issue 1 (December 2015)

• Record Type: Journal Article
• Authors: Li, Bo; Hulin, Michelle; Brain, Philip; Mansfield, John; Jackson, Robert; Harrison, Richard
• Is Part Of: Plant methods. Volume 11:Issue 1(2015)
• Page Start: 1
• Page End: 9
• Publication Date: 2015-12
• Subjects: Stem canker -- Artificial neural network -- Image analysis; Botany -- Methodology -- Periodicals; 572.2
• DOI: 10.1186/s13007-015-0100-8
• Languages: English
• ISSNs: 1746-4811

Abstract

Abstract Background Pseudomonas syringae can cause stem necrosis and canker in a wide range of woody species including cherry, plum, peach, horse chestnut and ash. The detection and quantification of lesion progression over time in woody tissues is a key trait for breeders to select upon for resistance. Results In this study a general, rapid and reliable approach to lesion quantification using image recognition and an artificial neural network model was developed. This was applied to screen both the virulence of a range ofP. syringae pathovars and the resistance of a set of cherry and plum accessions to bacterial canker. The method developed was more objective than scoring by eye and allowed the detection of putatively resistant plant material for further study. Conclusions Automated image analysis will facilitate rapid screening of material for resistance to bacterial and other phytopathogens, allowing more efficient selection and quantification of resistance responses.