H1N1 influenza vaccination in HIV-infected women on effective antiretroviral treatment did not induce measurable antigen-driven proliferation of the HIV-1 proviral reservoir. Issue 1 (December 2017)

• Record Type: Journal Article
• Authors: Wagner, Thor; Huang, Hannah; Salyer, Christen; Richardson, Kelly; Weinberg, Adriana; Nachman, Sharon; Frenkel, Lisa
• Is Part Of: AIDS research and therapy. Volume 14:Issue 1(2017)
• Page Start: 1
• Page End: 6
• Publication Date: 2017-12
• Subjects: HIV -- HIV DNA -- Influenza -- Vaccination -- H1N1 -- Pregnancy -- Antiretroviral therapy -- Latency -- Antiretroviral treatment; AIDS (Disease) -- Periodicals; 616.9792005
• DOI: 10.1186/s12981-017-0135-1
• Languages: English
• ISSNs: 1742-6405

Abstract

Abstract Objectives Antigen-induced activation and proliferation of HIV-1-infected cells is hypothesized to be a mechanism of HIV persistence during antiretroviral therapy. The objective of this study was to determine if proliferation of H1N1-specific HIV-infected cells could be detected following H1N1 vaccination. Methods This study utilized cryopreserved PBMC from a previously conducted trial of H1N1 vaccination in HIV-infected pregnant women. HIV-1 DNA concentrations and 437 HIV-1 C2V5env DNA sequences were analyzed from ten pregnant women on effective antiretroviral therapy, before and 21 days after H1N1 influenza vaccination. Results HIV-1 DNA concentration did not change after vaccination (median pre- vs. post-vaccination: 95.77 vs. 41.28 copies/million PBMC, p = .37). Analyses of sequences did not detect evidence of HIV replication or proliferation of infected cells. Conclusions Antigenic stimulation during effective ART did not have a detectable effect on the genetic makeup of the HIV-1 DNA reservoir. Longitudinal comparison of the amount and integration sites of HIV-1 in antigen-specific cells to chronic infections (such as herpesviruses) may be needed to definitively evaluate whether antigenic stimulation induces proliferation of HIV-1 infected cells.