Complete genome sequence of Pseudomonas stutzeri strain RCH2 isolated from a Hexavalent Chromium [Cr(VI)] contaminated site. Issue 1 (December 2017)

• Record Type: Journal Article
• Authors: Chakraborty, Romy; Woo, Hannah; Dehal, Paramvir; Walker, Robert; Zemla, Marcin; Auer, Manfred; Goodwin, Lynne; Kazakov, Alexey; Novichkov, Pavel; Arkin, Adam; Hazen, Terry
• Is Part Of: Standards in genomic sciences. Volume 12:Issue 1(2017)
• Page Start: 1
• Page End: 9
• Publication Date: 2017-12
• Subjects: Pseudomonas -- Nitrate reduction -- Chromium -- Hanford 100H; Genomics -- Periodicals; Genomics -- Periodicals; Genomics; Periodicals; 572.8605
• DOI: 10.1186/s40793-017-0233-7
• Languages: English
• ISSNs: 1944-3277

Abstract

Abstract Hexavalent Chromium [Cr(VI)] is a widespread contaminant found in soil, sediment, and ground water in several DOE sites, including Hanford 100 H area. In order to stimulate microbially mediated reduction of Cr(VI) at this site, a poly-lactate hydrogen release compound was injected into the chromium contaminated aquifer. Targeted enrichment of dominant nitrate-reducing bacteria post injection resulted in the isolation ofPseudomonas stutzeri strain RCH2.P. stutzeri strain RCH2 was isolated using acetate as the electron donor and is a complete denitrifier. Experiments with anaerobic washed cell suspension of strain RCH2 revealed it could reduce Cr(VI) and Fe(III). The genome of strain RCH2 was sequenced using a combination of Illumina and 454 sequencing technologies and contained a circular chromosome of 4.6 Mb and three plasmids. Global genome comparisons of strain RCH2 with six other fully sequencedP. stutzeri strains revealed most genomic regions are conserved, however strain RCH2 has an additional 244 genes, some of which are involved in chemotaxis, Flp pilus biogenesis and pyruvate/2-oxogluturate complex formation.