Complete genome sequence of Pseudoalteromonas phage vB_PspS-H40/1 (formerly H40/1) that infects Pseudoalteromonas sp. strain H40 and is used as biological tracer in hydrological transport studies. Issue 1 (December 2017)

• Record Type: Journal Article
• Authors: Kallies, René; Kiesel, Bärbel; Schmidt, Matthias; Kacza, Johannes; Ghanem, Nawras; Narr, Anja; Zopfi, Jakob; Wick, Lukas; Hackermüller, Jörg; Harms, Hauke; Chatzinotas, Antonis
• Is Part Of: Standards in genomic sciences. Volume 12:Issue 1(2017)
• Page Start: 1
• Page End: 7
• Publication Date: 2017-12
• Subjects: Pseudoalteromonas phage -- Siphoviridae -- AquaDiva -- Marine phage -- Virus -- Genome -- Bacteriophages as tracers; Genomics -- Periodicals; Genomics -- Periodicals; Genomics; Periodicals; 572.8605
• DOI: 10.1186/s40793-017-0235-5
• Languages: English
• ISSNs: 1944-3277

Abstract

Abstract Pseudoalteromonas phage vB_PspS-H40/1 is a lytic phage that infectsPseudoalteromonas sp. strain H40. Both, the phage and its host were isolated in the 1970s from seawater samples collected from the North Sea near the island of Helgoland, Germany. The phage particle has an icosahedral capsid with a diameter of ~43 to 45 nm and a long non-contractile tail of ~68 nm in length, a typical morphology for members of theSiphoviridae family. The linear dsDNA genome ofPseudoalteromonas phage vB_PspS-H40/1 has a sequence length of 45, 306 bp and a GC content of 40.6%. The genome has a modular structure and contains a high proportion of sequence information for hypothetical proteins, typically seen in phage genome sequences. This is the first report of the complete genome sequence of this lytic phage, which has been frequently used since the 1990s as biological tracer in hydrogeological transport studies.