Biallelic modification of IL2RG leads to severe combined immunodeficiency in pigs. Issue 1 (December 2016)

• Record Type: Journal Article
• Authors: Kang, Jung-Taek; Cho, Bumrae; Ryu, Junghyun; Ray, Caitlin; Lee, Eun-Jin; Yun, Yun-Jin; Ahn, SunMi; Lee, JinSeok; Ji, Dal-Young; Jue, Nathaniel; Clark-Deener, Sherrie; Lee, Kiho; Park, Kwang-Wook
• Is Part Of: Reproductive biology and endocrinology. Volume 14:Issue 1(2016)
• Page Start: 1
• Page End: 9
• Publication Date: 2016-12
• Subjects: IL2RG -- Severe Combined Immunodeficiency (SCID) -- CRISPR/Cas9 -- Somatic cell nuclear transfer (SCNT) -- Knockout pigs; Reproduction -- Periodicals; Generative organs -- Pathophysiology -- Periodicals; Reproduction -- Endocrine aspects -- Periodicals; Endocrine glands -- Diseases -- Periodicals; Embryology -- Periodicals; 571.8
• DOI: 10.1186/s12958-016-0206-5
• Languages: English
• ISSNs: 1477-7827

Abstract

Abstracts Background Pigs with SCID can be a useful model in regenerative medicine, xenotransplantation, and cancer cell transplantation studies. Utilizing genome editing technologies such as CRISPR/Cas9 system allows us to generate genetically engineered pigs at a higher efficiency. In this study, we report generation and phenotypic characterization ofIL2RG knockout female pigs produced through combination of CRISPR/Cas9 system and SCNT. As expected, pigs lacking IL2RG presented SCID phenotype. Methods First, specific CRISPR/Cas9 systems targetingIL2RG were introduced into developing pig embryos then the embryos were transferred into surrogates. A total of six fetuses were obtained from the embryo transfer and fetal fibroblast cell lines were established. ThenIL2RG knockout female cells carrying biallelic genetic modification were used as donor cells for SCNT, followed by embryo transfer. Results Three live cloned female piglets carrying biallelic mutations inIL2RG were produced. All cloned piglets completely lacked thymus and they had a significantly reduced level of mature T, B and NK cells in their blood and spleen. Conclusions Here, we generatedIL2RG knockout female pigs showing phenotypic characterization of SCID. ThisIL2RG knockout female pigs will be a promising model for biomedical and translational research.