Accuracy of preimplantation genetic screening (PGS) is compromised by degree of mosaicism of human embryos. Issue 1 (December 2016)

• Record Type: Journal Article
• Authors: Gleicher, Norbert; Vidali, Andrea; Braverman, Jeffrey; Kushnir, Vitaly; Barad, David; Hudson, Cynthia; Wu, Yang-Guan; Wang, Qi; Zhang, Lin; Albertini, David
• Is Part Of: Reproductive biology and endocrinology. Volume 14:Issue 1(2016)
• Page Start: 1
• Page End: 7
• Publication Date: 2016-12
• Subjects: Preimplantation genetic screening (PGS) -- In vitro fertilization (IVF) -- Embryos -- Embryo mosaicism -- Trophectoderm biopsy -- Blastocyst; Reproduction -- Periodicals; Generative organs -- Pathophysiology -- Periodicals; Reproduction -- Endocrine aspects -- Periodicals; Endocrine glands -- Diseases -- Periodicals; Embryology -- Periodicals; 571.8
• DOI: 10.1186/s12958-016-0193-6
• Languages: English
• ISSNs: 1477-7827

Abstract

Abstract Background To preclude transfer of aneuploid embryos, current preimplantation genetic screening (PGS) usually involves one trophectoderm biopsy at blastocyst stage, assumed to represent embryo ploidy. Whether one such biopsy can correctly assess embryo ploidy has recently, however, been questioned. Methods This descriptive study investigated accuracy of PGS in two ways. Part I: Two infertile couples donated 11 embryos, previously diagnosed as aneuploid and, therefore, destined to be discarded. They were dissected into 37 anonymized specimens, and sent to another national laboratory for repeat analyses to assess (i) inter-laboratory congruity and (ii) intra-embryo congruity of multiple embryo biopsies in a single laboratory. Part II: Reports on human IVF cycle outcomes after transfer of allegedly aneuploid embryos into 8 infertile patients. Results Only 2/11 (18.2 %) embryos were identically assessed at two PGS laboratories; 4/11 (36.4 %), on repeat analysis were chromosomally normal, 2 mosaic normal/abnormal, and 5/11 (45.5 %) completely differed in reported aneuploidies. In intra-embryo analyses, 5/10 (50 %) differed between biopsy sites. Eight transfers of previously reported aneuploid embryos resulted in 5 chromosomally normal pregnancies, 4 delivered and 1 ongoing. Three patients did not conceive, though 1 among them experienced a chemical pregnancy. Conclusions Though populations of both study parts are too small to draw statistically adequately powered conclusions on specific degrees of inaccuracy of PGS, here presented results do raise concerns especially about false-positive diagnoses. While inter-laboratory variations may at least partially be explained by different diagnostic platforms utilized, they cannot explain observed intra-embryo variations, suggesting more frequent trophectoderm mosiaicsm than previously reported. Together with recentl published mouse studies of lineages-specific degrees of survival of aneuploid cells in early stage embryos, these results call into question the biological basis of PGS, based on the assumption that a single trophectoderm biopsy can reliably determine embryo ploidy.