High‐risk human papillomavirus detection in oropharyngeal cancers: Comparison of saliva sampling methods. Issue 5 (23rd December 2018)

• Record Type: Journal Article
• Authors: Tang, Kai Dun; Kenny, Liz; Frazer, Ian H.; Punyadeera, Chamindie
• Is Part Of: Head & neck. Volume 41:Issue 5(2019)
• Page Start: 1484
• Page End: 1489
• Publication Date: 2018-12-23
• Subjects: human papillomavirus -- oropharyngeal cancer and noninvasive biomarker -- physical status -- salivary oral rinse -- unstimulated whole mouth saliva; Head -- Diseases -- Periodicals; Neck -- Diseases -- Periodicals; Head -- Periodicals; Neck -- Periodicals; Face -- Periodicals; 617.51059
• DOI: 10.1002/hed.25578
• Languages: English
• ISSNs: 1043-3074

Abstract

Abstract: Background: Accumulating evidence has suggested the utility of salivary oral rinse as a diagnostic fluid to detect oral human papillomavirus (HPV) DNA, but there are many methods for collecting saliva. Methods: Salivary oral rinse and unstimulated whole mouth saliva samples were collected from 45 oropharyngeal cancer (OPC) patients. Results: We show a positive correlation of HPV‐16 E2 ( r = 0.95, P < 0.0001) and E6/7 ( r = 0.93, P < 0.0001) relative copy number as well as HPV genotypes in both sample methods. There was a significant correlation between the two sample methods in the ratio of HPV16 E2 to E6/7 DNA ( r = 0.46, P < 0.01). Consistent with previous studies, a mixed HPV‐16 form (episomal and integrated) was commonly found in both saliva and tumor samples. Conclusion: Detection of HPV in saliva samples collected by either method yielded comparable results, and showed good sensitivity for detection of HPV derived from OPC.