The Secretome of Microglia Regulate Neural Stem Cell Function. (1st May 2019)

• Record Type: Journal Article
• Authors: Osman, Ahmed M.; Rodhe, Johanna; Shen, Xianli; Dominguez, Cecilia A.; Joseph, Bertrand; Blomgren, Klas
• Is Part Of: Neuroscience. Volume 405(2019)
• Page Start: 92
• Page End: 102
• Publication Date: 2019-05-01
• Subjects: FBS fetal bovine serum -- IL-4 interleukin-4 -- LDH lactate dehydrogenase -- LPS lipopolysaccharide -- NSCs neural stem cells -- SVZ subventricular zone -- TNFα tumor necrosis factor α; M1 -- M2 -- Neurogenesis -- Neuroinflammation; Neurochemistry -- Periodicals; Neurophysiology -- Periodicals; Neurology -- Periodicals; Neurochimie -- Périodiques; Neurophysiologie -- Périodiques; Neurochemistry; Neurophysiology; Electronic journals; Periodicals; Electronic journals; 612.8
• DOI: 10.1016/j.neuroscience.2017.10.034
• Languages: English
• ISSNs: 0306-4522

Abstract

Highlights: Pro-inflammatory microglia negatively regulate NSCs survival. Anti-inflammatory microglia increase NSC migration. Anti-inflammatory microglia induce less astrocytic differentiation. Microglia subtypes down-regulate factors associated with neuronal differentiation. Pro-inflammatory microglia induce higher expression of CCL2 in differentiated NSCs. Abstract: Brain injury is associated with neuroinflammation, and microglia are key players in this process. Microglia can acquire pro-inflammatory or anti-inflammatory properties, but how this affects neural stem cells (NSCs) remains controversial. Here, NSCs were grown in conditioned media collected from either non-stimulated microglia, or microglia stimulated with pro- or anti-inflammatory agents. NSC survival, proliferation, migration, and differentiation were investigated thereafter. We found that NSCs kept in conditioned medium from the anti-inflammatory microglial subtype had better survival, increased migration, and lower astrocytic differentiation compared to NSCs grown in conditioned medium collected from the pro-inflammatory subtype. Finally, we found that NSCs differentiated in microglial conditioned media generated cells expressing the pro-inflammatory chemokine CCL2, most pronounced when differentiated in medium from the pro-inflammatory microglia subtype. Our results show that microglial subtypes regulate NSCs differently and induce generation of cells with inflammatory properties.