NK cell immune responses differ after prime and boost vaccination. Issue 5 (22nd February 2019)

• Record Type: Journal Article
• Authors: Palgen, Jean‐Louis; Tchitchek, Nicolas; Huot, Nicolas; Elhmouzi‐Younes, Jamila; Lefebvre, Cécile; Rosenbaum, Pierre; Dereuddre‐Bosquet, Nathalie; Martinon, Frédéric; Hocini, Hakim; Cosma, Antonio; Müller‐Trutwin, Michaela; Lévy, Yves; Le Grand, Roger; Beignon, Anne‐Sophie
• Other Names: Santos‐Argumedo Leopoldo guestEditor.; Pelayo Rosana guestEditor.; Justement Lou guestEditor.; Schnoor Michael guestEditor.
• Is Part Of: Journal of leukocyte biology. Volume 105:Issue 5(2019)
• Page Start: 1055
• Page End: 1073
• Publication Date: 2019-02-22
• Subjects: innate lymphoid immunity -- mass cytometry -- MVA -- NHP -- NK cells -- prime‐boost -- vaccination -- transcriptomics; Leucocytes -- Periodicals; Reticulo-endothelial system -- Periodicals; 571.96
• DOI: 10.1002/JLB.4A1018-391RR
• Languages: English
• ISSNs: 0741-5400

Abstract

Abstract: A better understanding of innate responses induced by vaccination is critical for designing optimal vaccines. Here, we studied the diversity and dynamics of the NK cell compartment after prime‐boost immunization with the modified vaccinia virus Ankara using cynomolgus macaques as a model. Mass cytometry was used to deeply characterize blood NK cells. The NK cell subphenotype composition was modified by the prime. Certain phenotypic changes induced by the prime were maintained over time and, as a result, the NK cell composition prior to boost differed from that before prime. The key phenotypic signature that distinguished NK cells responding to the boost from those responding to the prime included stronger expression of several cytotoxic, homing, and adhesion molecules, suggesting that NK cells at recall were functionally distinct. Our data reveal potential priming or imprinting of NK cells after the first vaccine injection. This study provides novel insights into prime‐boost vaccination protocols that could be used to optimize future vaccines. Abstract : NK cells subsets are modulated by the first vaccine encounter and thus respond differently to boost immunization.