Sex-linked AFLP marker identification in dioecious Betelvine (Piper betle L.). Issue 4 (4th July 2019)

• Record Type: Journal Article
• Authors: Goyat, Shalini; Grewal, Anita; Singh, Devendra; Katiyar, R.S.; Tewari, S.K.; Nainwal, R.C; Bindu, K. Hima
• Is Part Of: Journal of horticultural science & biotechnology. Volume 94:Issue 4(2019)
• Page Start: 422
• Page End: 427
• Publication Date: 2019-07-04
• Subjects: AFLP -- dioecious -- sex-specific marker -- P. betle -- molecular marker -- betelvine; Horticulture -- Periodicals; Plant biotechnology -- Periodicals; Biotechnology -- Periodicals; Biotechnology; Horticulture; Plant biotechnology; Periodicals; 635.05
• DOI: 10.1080/14620316.2018.1538707
• Languages: English
• ISSNs: 1462-0316

Abstract

ABSTRACT: This study aimed to develop sex-specific AFLP markers for dioecious Piper betle (Betelvine). A combination of ulk segregant analysis (BSA) and non-radioactive labelling led to simple, rapid and reliable PCR-based screening of sex-specific markers. A total of nine primer combinations were screened using BSA for male and female cultivars. Seven out of nine primer combinations produced bands specific to either of the sex. These seven primer combinations when used for screening DNA sample from individual male and female cultivars yielded two primer combinations which produced male sex-specific markers. Primer combinations Eco RI-CTA/ Mse I-CTA and Eco RI-CGT/ Mse I-CGT produced unique fragments of ~ 147 bp and ~ 326 bp, respectively, in bulk DNA as well as in DNA sample from individual male and female cultivars. These unique fragments were present in all the tested males and absent from all the tested female cultivars.