Antifungal-resistant Mucorales in different indoor environments. Issue 2 (3rd April 2019)

• Record Type: Journal Article
• Authors: Caetano, Liliana Aranha; Faria, Tiago; Springer, Jan; Loeffler, Juergen; Viegas, Carla
• Is Part Of: Mycology. Volume 10:Issue 2(2019)
• Page Start: 75
• Page End: 83
• Publication Date: 2019-04-03
• Subjects: Mucorales -- antifungal drug-resistance -- indoor environments; Fungi -- Periodicals; Mycology -- Periodicals; Fungi; Mycology; Periodicals; 579.505
• DOI: 10.1080/21501203.2018.1551251
• Languages: English
• ISSNs: 2150-1203

Abstract

ABSTRACT: This paper sought to address the prevalence of Mucorales in different indoor environments in Portugal. Environmental samples (183 in total) were collected at dwellings ( n = 79) and workplaces (bakeries, swine farms, taxis, waste-sorting plants) ( n = 93) by passive sampling using electrostatic dust collector (EDC), air-conditioning filters, litter, and/or raw materials. Samples were inoculated onto non-selective MEA and DG18 media and were screened for antifungal drug-resistance in azole-supplemented agar Sabouraud media. A probe-based Mucorales-specific real-time PCR assay (Muc18S) was used to detect Mucorales in complement to conventional culture-based methods. Mucorales order was found as more prevalent in air-conditioning filters from waste-sorting fork lifters (35.7%). Amongst Mucorales isolates able to grow in azole-supplemented media, 16 isolates of Mucor sp., Rhizopus sp. or Rhizomucor sp. were not susceptible to 1 mg/L voriconazole, and four isolates of Mucor sp. or Rhizopus sp. were not susceptible to 4 mg/L itraconazole. In conclusion, combination of the culture-based and molecular methods proved to be reliable for Mucorales order identification in complex environmental samples.