A preliminary DNA barcode selection for the genus Russula (Russulales, Basidiomycota). Issue 2 (3rd April 2019)

• Record Type: Journal Article
• Authors: Li, Guo-Jie; Zhao, Rui-Lin; Zhang, Chu-Long; Lin, Fu-cheng
• Is Part Of: Mycology. Volume 10:Issue 2(2019)
• Page Start: 61
• Page End: 74
• Publication Date: 2019-04-03
• Subjects: Barcode gap -- fungal identification -- intra-and inter-specific variation -- Russulaceae -- species recognition; Fungi -- Periodicals; Mycology -- Periodicals; Fungi; Mycology; Periodicals; 579.505
• DOI: 10.1080/21501203.2018.1500400
• Languages: English
• ISSNs: 2150-1203

Abstract

ABSTRACT: Russula is a worldwid genus which has a high species diversity . Aiming accurate and rapid species identification, candidate genes nLSU (28S), ITS, tef-1α, mtSSU, rpb1, and rpb2, were analysed as potential DNA barcodes. This analysis included 433 sequences from 38 well-circumscribed Russula species of eight subgenera. Two vital standards were analysed for success species identification using DNA barcodes, specifically inter- and intra-specific variations together with the success rates of PCR amplification and sequencing. Although the gap between inter- and intra-specific variations was narrow, ITS met the qualification standards for a target DNA barcode. Overlapping inter- and intra-specific pairwise distances were observed in nLSU, tef-1α, mtSSU, and rpb2 . The success rates of PCR amplification and sequencing in mtSSU and rpb1 were lower than those of others. Gene combinations were also investigated for resolution of species recognition. ITS-rpb2 was suggested as the likely target DNA barcode for Russula, owing to the two viatal standards above. Since nLSU has the lowest minimum of inter-specific variation, and tef-1α has the highest overlap between intra- and inter-species variations among the candidate genes, they are disqualified from the selection for DNA barcode of Russula .