Transcriptional status of mouse oocytes corresponds with their ability to generate Ca2+ release. Issue 5 (May 2019)

• Record Type: Journal Article
• Authors: Fluks, Monika; Szczepanska, Katarzyna; Ishikawa, Takao; Ajduk, Anna
• Is Part Of: Reproduction. Volume 157:Issue 5(2019)
• Page Start: 465
• Page End: 474
• Publication Date: 2019-05
• Subjects: Reproduction -- Periodicals; Reproduction -- Molecular aspects -- Periodicals; Reproduction -- Immunological aspects -- Periodicals; Reproduction -- Endocrine aspects -- Periodicals; Fertility -- Periodicals; Human reproduction -- Periodicals; 571.805
• DOI: 10.1530/REP-18-0625
• Languages: English
• ISSNs: 1470-1626

Abstract

Abstract : In fully grown ovarian follicles both transcriptionally active (NSN) and inactive (SN) oocytes are present. NSN oocytes have been shown to display lower developmental potential. It is possible that oocytes that have not completed transcription before meiosis resumption accumulate less RNA and proteins required for their further development, including those responsible for regulation of Ca 2+ homeostasis. Oscillations of the cytoplasmic concentration of free Ca 2+ ions ([Ca 2+ ]i ) are triggered in oocytes by a fertilizing spermatozoon and are crucial for inducing and regulating further embryonic development. We showed that NSN-derived oocytes express less inositol 1, 4, 5-triphosphate receptor type 1 (IP3R1), store less Ca 2+ ions and generate weaker spontaneous [Ca 2+ ]i oscillations during maturation than SN oocytes. Consequently, NSN oocytes display aberrant [Ca 2+ ]i oscillations at fertilization. We speculate that this defective regulation of Ca 2+ homeostasis might be one of the factors responsible for the lower developmental potential of NSN oocytes.