Genomic and transcriptomic comparison between Staphylococcus aureus strains associated with high and low within herd prevalence of intra-mammary infection. Issue 1 (December 2017)

• Record Type: Journal Article
• Authors: Capra, E.; Cremonesi, P.; Pietrelli, A.; Puccio, S.; Luini, M.; Stella, A.; Castiglioni, B.
• Is Part Of: BMC microbiology. Volume 17:Issue 1(2017)
• Page Start: 1
• Page End: 16
• Publication Date: 2017-12
• Subjects: Staphylococcus aureus -- Mastitis -- Virulence -- Genome -- Transcriptome -- Next generation sequencing; Microbiology -- Periodicals; 579.05
• DOI: 10.1186/s12866-017-0931-8
• Languages: English
• ISSNs: 1471-2180

Abstract

Abstract Background Staphylococcus aureus (Staph. aureus ) is one of the major pathogens causing mastitis in dairy ruminants worldwide. The chronic nature ofStaph. aureus infection enhances the contagiousness risk and diffusion in herds. In order to identify the factors involved in intra-mammary infection (IMI) and diffusion in dairy cows, we investigated the molecular characteristics of two groups ofStaph. aureus strains belonging to ST8 and ST398, differing in clinical properties, through comparison of whole genome and whole transcriptome sequencing. Results The two groups of strains, one originated from high IMI prevalence herds and the other from low IMI prevalence herds, present a peculiar set of genes and polymorphisms related to phenotypic features, such as bacterial invasion of mammary epithelial cells and host adaptation. Transcriptomic analysis supports the high propensity of ST8 strain to chronicity of infection and to a higher potential cytotoxicity. Conclusions Our data are consistent with the invasiveness and host adaptation feature for the strains GTB/ST8 associated to high within-herd prevalence of mastitis. Variation in genes coding for surface exposed proteins and those associated to virulence and defence could constitute good targets for further research.